Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Fisetin Inhibits Osteoclast Differentiation via Downregulation of p38 and c-Fos-NFATc1 Signaling Pathways

doi: 10.1155/2012/810563

Figure Lengend Snippet: Fisetin inhibits osteoclastic bone resorption. (a) Effect of fisetin on mRNA expression of cathepsin K was analyzed by real-time PCR as described in . ** P < 0.01; *** P < 0.001 (versus “the vehicle control”). (b) Attached cells on BioCoat Osteologic MultiTest slides were removed and photographed under a light microscope (bottom images). Pit areas were quantified using ImageJ program (upper graph). ** P < 0.01; *** P < 0.001 (versus “the control”). (c) Mature osteoclasts were plated on BioCoat slides and treated for 24 h with the indicated concentrations of fisetin. Cells were fixed, permeabilized, and stained with TRAP. TRAP-positive multinucleated cells were counted (upper graph) and photographed under a light microscope (bottom images).

Article Snippet: Co-cultured cells were detached from the collagen-coated dishes, replated on BioCoat Osteologic MultiTest slides in 96-well plates, and treated with fisetin for 24 h. The cells on the BioCoat slides were stained for TRAP, and photographs were taken under a light microscope at 40x magnification.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Light Microscopy, Staining